Tumor-Derived Microvesicles Enhance Cross-Processing Ability of Clinical Grade Dendritic Cells

Tumor-Derived Microvesicles Modulate Antigen Cross-Processing via Reactive Oxygen Species-Mediated Alkalinization of Phagosomal Compartment in Dendritic Cells

Dendritic cells (DCs) are the only antigen-presenting cells able to prime naïve T cells and cross-prime antigen-specific CD8+ T cells. Their functionality is a requirement for the induction and maintenance of long-lasting cancer immunity. Albeit intensively investigated, the in vivo mechanisms underlying efficient antigen cross-processing and presentation are not fully understood. Several piece...

DIFFERENTIATION OF MONOCYTE DERIVED DENDRITIC CELLS IN SERUM FREE CONDITIONS

Human peripheral blood monocytes (HPBM) were cultured in the absence of human serum and were converted into a state exhibiting a high accessory function expressed by their ability of supporting lymphocyte proliferation. After a prolonged culture in serum free media the monocyte derived cells were highly viable, increased in size and developed veils and dendritiform elongatio'l1s. Paralleli...

NADPH oxidase-derived reactive oxigen species enhance antigen cross-presentation of dendritic cells

comparative proteomic analysis of tumor mesenchymal-like cells derived from high grade vs. low grade gliomas

objective: gliomas are the most common primary brain tumors, and have been ranked as the fourth leading cause of cancer death. tumor mesenchymal-like stem cells (tmscs) contribute to the aggressive behavior of glial tumors by providing a favorable microenvironment for the malignant cells. the aims of our study were to identify differential proteome of tmscs derived from low vs. high grade gliom...

Multiple myeloma cell-derived microvesicles are enriched in CD147 expression and enhance tumor cell proliferation

Multiple myeloma (MM) is characterized by the clonal expansion of malignant plasma cells within the bone marrow. There is a growing literature that tumor cells release biologically active microvesicles (MVs) that modify both local and distant microenvironments. In this study, our goals were to determine if MM cells release MVs, and if so, begin to characterize their biologic activity. Herein we...